Production of Acetone, Butanol, and Ethanol in Repeated Batch Culture from Tryptone-Yeast Extract-Acetate Medium by Clostridium acetobutylicum YM1

Abstract

in this study, the use of a low-cost fermentation medium has a significant impact on the viability of most fermentation processes. A local strain of Clostridium acetobutylicum YM1 was used to repeatedly batch culture acetone-butanol-ethanol (ABE). Use of the tryptone-yeast extract-acetate (TYA) medium, which contains 30 g/L glucose, was made for batch and repeated batch cultures. The experiment discovered the highest acetone-butanol-ethanol (ABE) concentration in repeated batch culture. ABE production during repeated batch fermentation was examined for the effects of several parameters, including drain and fill volume of medium and drain and fill times. The findings of repeated batch fermentation demonstrated that the production was maximum at 50% (v/v) of drain and fill volume at 48 hours acetone, butanol, and ethanol (ABE). The second cycle had the highest concentrations of butanol, ethanol, and acetone at 7.12, 2.39, and 3.04 g/L, respectively. This study demonstrated that Clostridium acetobutylicum YM1 batch culture fermentation of acetone-butanol-ethanol (ABE) was improved by repeated batch fermentation. The TYA medium can be thought of as a viable low cost substrate for ABE fermentation, according to this study's findings.